Comparative evaluation of multiple protein extraction procedures from three species of the genus Caulerpa

Abstract The aim of this study was to define the simplest and least expensive protocol for total protein extraction three different macroalgae genus Caulerpa (the invasive C. taxifolia cylindracea autochthonous prolifera ). Five multi-step procedures, set up other macroalgal species, were tested. For each them, pre-treatment conditions simultaneously examined, according a factorial design, considering starting material, solvent-to-biomass ratio, incubation temperature. Protein yield in obtained extracts estimated with Bradford method. Further, polyacrylamide gel electrophoresis (SDS-PAGE) used resolve proteins, assessing their quality integrity. Significant differences observed among protocols tested, also relation considered species. Profiles having an acceptable , from results, best method obtain high two above-mentioned species appears require use primary TCA/acetone buffer followed by lysis NaCl, KCl, urea, Triton, SDS protease inhibitor. particular, fresh pulped material buffer-to-biomass ratio 10:1 temperature 4°C. instead, none tested produced satisfactory results further studies will be required.